Thursday , February 25 2021

DNA with a twist: Discovery can do more antibiotic drug development

DNA replication is important for all living conditions, but in some organisms it can be prevented by passing in the DNA sequence, & # 39; supercoils. If too many supercoils are being set, TV shows will underestimate TV stress.

A molecular machine called DNA gyrase, found in bacterial cells but not human cells, loses the desire to earn DNA replication to normal, but so far it was understands the idea of ​​how this really happens in real life numbers.

The process is especially important for drug developers, DNA gyros will be successfully interrupted when it works to find stopper in bacterial DNA cells, the bacteria will die and the infectious disadvantage of the host will be prevented.

The York University team, in collaboration with the John Innes Center, Oxford and the Adam Mickiewicz University, in Poland, uses a special laser microscope to light a fluorescence protein that makes DNA gyrase gloss. This would show scientists in bacterial inland and, first, observe how the molecular machine is present in DNA.

Professor Mark Leake, of physiology and physics departmental physics; the University of York said: "With the use of modified fluorescent proteins, the DNA gyrase can be made to glow, while the cellular machine used for DNA replication can be used with a variety of reddish- glomerate protein.

"These different colors can then be divided into different detector channels to determine the exact location of DNA gyras to determine the exact point in which DNA replication is actually inserted into a single libbacterial cell."

The researchers have discovered that the DNA gyrase has its twofold activity justified for the point in which DNA in a cell responds.

Professor Leake said, "The molecular machines DNA DNA replication has passed through the DNA, but this work can create a small nanoscale of DNA that will collect it for the replication machine, as well as tangential cables on & # 39; the background of your TV set.

"We have now indicated that various DNA gyrase molecules actively bind to a zone directly to the replication and relate the DNA nano acceleration faster than the replication machine moved to the DNA.

"Maybe a" twist barrier "from rebuilding, which can stop rehearsal machines from scrambling to DNA, stop replication, and kill the cell."

DNA gyrase is a goal for a number of different antibiotics, but with several super-bugs & # 39; They have been resistant to antibiotics, there is more urgent need to understand how bactericides work in real time.

Professor Leake said, "Now that we know how DNA gyrase realizes the role of bacterial life, we can help with the design of new types of drugs that can work out DNA gyras, # 39; t medicines can be more directed and possibly dead. Dangerous bacterial infections in people.

"Human cells have similar mechanisms to resolve DNA twist but different molecular mechanisms, and our work on DNA gyrase in bacteria give us valuable insights into generalized mechanisms that control the operation of this class of remarkable biomolecule for all organisms. "

The study is published in the journal Nucleic Acids Research.

This article has been replaced by material that comes from the University of York. Note: material can be changed for length and content. For further information, please contact the so-called source.

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